Device
Part:BBa_K797005:Design
Designed by: Tomohiro Nobeyama Group: iGEM12_Kyoto (2012-09-20)
T7 R9 GFP DT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 986 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 987
Illegal PstI site found at 1001
Illegal NotI site found at 7
Illegal NotI site found at 994 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 987 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 987
Illegal PstI site found at 1001 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 772
Design Notes
Source
References
[1]Microsugar Chang et al. (2005)"Cellular internalization of fluorescent proteins via arginine-rich intracellular delivery peptide in plant cells" Plant Cell Physiol, 46(3), 482–488